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. 2023 Jul 14;16:1115880. doi: 10.3389/fnmol.2023.1115880

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Copyright © 2023 Daniel, Elizarova, Shaib, Chouaib, Magnussen, Wang, Brose, Rhee and Tirard.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nacc1 interacts with the synaptic proteins SynGAP1 and GluK2A. (A) Anti-HA (left panel) and anti-SynGAP1 (right panel) Western blot analysis of input and eluate fractions from an anti-SynGAP1 affinity purification (IP: anti-SynGAP1) in N2A cells expressing SynGAP1 and HA-Nacc1 alone or in combination. Black arrowheads indicate unmodified HA-Nacc1 (left) or SynGAP1 (right). (B) Anti-GluK2A/3 (left panel) and anti-HA (right panel) Western blot analysis of input and eluate fractions from an anti-HA affinity purification (IP: anti-HA) in N2A cells expressing GluK2A and HA-Nacc1 alone or in combination. A black arrowhead indicates GluK2A in the left blot. On the right, a black arrowhead indicates unmodified HA-Nacc1. Molecular weight markers (kDa) are indicated to the left of the blots. Individual lanes of single Western blot membranes are shown in A and B, since several lanes contained samples not relevant to the figure. The full blots are available in Supplementary Figure S8. Images are representative of at least 3 independent experiments.