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. 2023 Jul 14;16:1115880. doi: 10.3389/fnmol.2023.1115880

Table 1.

List of validated Nacc1 interaction partners.

Protein name Abbreviation # of Clones
Unique Total
Zinc finger MYM-type 2 Zmym2 4 4
Protein inhibitor of activated STAT 2* Pias2 3 12
Ring Finger protein 111* RNF111 2 25
Nucleus acumbens associated protein 1 Nacc1 2 4
Zinc finger and BTB domain 18* zBTB18 2 4
L3MBTL2 polycomb repressive complex 1 subunit L3MBTL2 2 2
Synaptic Ras GTPase activating protein 1* SynGAP1 2 2
CCHC domain containing 18* Zcchc18 1 10
Protein inhibitor of activated STAT 1* Pias1 1 3
Caspase 8 associated protein 2* casp8ap2 1 2
Protein inhibitor of activated STAT 3* Pias3 1 1
Polo-like kinase 2* Plk2 1 1
RAN binding protein 2* Ranbp2 1 1
Histone acetyltransferase KAT5* Kat5 1 1
Activating transcription factor 7-interacting protein* Atf7ip 1 1
G-protein coupled receptor associated sorting 1* gprasp1 1 1

Names of the prey clones identified and validated in the Nacc1 yeast two-hybrid screen. Previously unreported interaction partners are indicated (*). All prey fragments of the listed proteins were positive for both reporter genes (HIS3 and LacZ). The identity of these proteins was determined using the Basic Local Alignment Search tool (BLAST) and the GenBank database (NCBI). The number of total clones indicates the number of prey fragments isolated in total, while the number of unique clones indicates the number of prey clones that encoded distinct sequences within the protein.