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. 2023 Jun 1;37(11-12):490–504. doi: 10.1101/gad.350569.123

Figure 2.

Figure 2.

Nkx2.2SDmut/Cre embryos show an expansion of other endocrine cell types at the expense of β cells beginning at E15.5. (A–E) Characterization of insulin (INS+), glucagon (GCG+), ghrelin (GHR+), and somatostatin (SST+) cells in Nkx2.2SDmut/Cre (SDmut/Cre) and Nkx2.2Cre/+ (WT/Cre) embryos. (A,B) Immunostaining (A) and quantification (B) of endocrine cell types in E15.5 embryos. (C–E) Immunostaining (C), quantification (D), and qRT-PCR analysis (E) at E18.8. Data are presented as mean ± SEM. (ns) Not significant, (*) P < 0.05, (**) P < 0.01, (***) P < 0.001. Scale bars represent 50 µm.