TABLE 5.
Divergence within E1 and E2a
| Animal | Protein | No. of nt analyzed | No. of nt changes | Rate of variation (10−3)b | No. of aa analyzed | No. of aa changes | aa/nt (%)c |
|---|---|---|---|---|---|---|---|
| x174 | E1 | 576 | 10 | 2.2 | 192 | 4 | 40 |
| E2 | 1,089 | 14 | 1.6 | 363 | 4 | 29 | |
| HVR-1 | 90 | 1 | 1.4 | 30 | 1 | 100 | |
| x304 | E1 | 576 | 9 | 2.1 | 192 | 4 | 44 |
| E2 | 1,089 | 19 | 2.4 | 363 | 4 | 21 | |
| HVR-1 | 90 | 0 | 0 | 30 | 0 | 0 |
a
The E1 and E2 regions analyzed spanned aa 192 to 383 (576 nucleotides [nt]) and an 384 to 746 (1,089 nucleotides), respectively. The HVR-1 region spanned aa 384 to 413 (90 nucleotides). The duration of time between the collection of serum samples was 7.9 years for x174 and 7.4 years for x304.
b
The rate of variation was calculated as the number of nucleotide changes/site/years between temporally spaced bleeds.
c
The percentage of nucleotide changes resulting in amino acid changes.