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. 2023 Mar 21;29(8):1327–1341. doi: 10.1093/ibd/izad024

Figure 4.

Figure 4.

Integrin β7 deficient IL-10-/- mice and MAdCAM-1 blockade lower fecal IgA and worsens colitis. A, Colonic LP IgA+ ASCs counts of indicated strains at 8 to 12 weeks of age, determined by flow cytometry (non-inflamed [N/I], inflamed [I], as determined by histological scores; total histological scores 0-1 represent N/I mice, scores >1 represent inflamed mice). Freshly isolated LP cells were stained, and events gated on live single cells. B, Fecal IgA of the indicated mouse strains. Expression was normalized to total fecal protein. C, Representative colonic histology and colonic and cecal histological indices. All data are presented as mean ±SD, from n > 9 mice in each data set. Each data point represents an individual mouse. Statistical significance was determined by ANOVA, followed by Tukey multiple comparison test. D, Serial fecal IgA levels of anti-MAdCAM-1-treated IL-10-/- mice. E, colonic and cecal histological indices of treated mice at week 8. F, Fecal bacterial counts from IL-10-/- mice treated with isotype or MECA-367, determined via RT-qPCR for 16S rRNA expression. All data are presented as mean ±SD, from n > 10 mice in each data set. Statistical significance was determined by Student t test (B), or 2-way ANOVA, followed by Sidak multiple comparison test. From Tyler et al.63