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. 2023 Jun 8;8(11):e166001. doi: 10.1172/jci.insight.166001

Figure 3. Silencing REST relieves ferroptosis under HR condition.

Figure 3

HK2 cells were transfected with control or siRNAs against REST (siREST), and then exposed to HR injury for RNA-seq (n = 4). (A–D) PI/calcein-AM staining (A), KEGG pathway classification (B), GO enrichment analysis of significant changes in cellular component (C), and GSEA enrichment analysis of ferroptosis pathway (D) between control and REST-knockdown group under HR condition. Scale bar: 50 μm. (E–I) Representative TEM images of mitochondria (E), GSH levels (F), MDA levels (G), lipid ROS production (H), and ROS production (I) in different groups (n = 3). Scale bar: 1 μm (left), 0.15 μm (right). N, nucleus. Data are shown as mean ± SD and were analyzed by 1-way ANOVA (A and F–I). *P < 0.05; **P < 0.01; ***P < 0.001.