Figure 6. Renal tubule–specific knockout of Rest alleviates lipid peroxidation in IRI-induced AKI mice.

Rest^RTKO and Rest^fl/fl mice were subjected to IRI and sacrificed on reperfusion day 1. Ferrostatin-1 (Fer-1; 5 mg/kg) or normal saline was intraperitoneally injected into the mice at the onset of reperfusion. The following indices were evaluated (n = 8 mice per group). (A and B) H&E staining (A) and injury scores (B) of the kidneys from all groups. *, cortex; #, S3 of the proximal tubules. Scale bars: 1.25 mm (left) and 50 μm (middle and right). (C and D) Levels of Scr (C) and BUN (D) from mice in A. (E and F) GSH levels (E) and MDA levels (F) of the kidneys from mice in A. Data are shown as mean ± SD and were analyzed by 1-way ANOVA (B–F). *P < 0.05; **P < 0.01; ***P < 0.001.