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. 2023 Jun 8;8(11):e165373. doi: 10.1172/jci.insight.165373

Figure 2. MOG IgG1 and IgG3 induce CDC while all IgG subclasses induce ADCP.

Figure 2

The MOG mAb variable region was subcloned into Fc vectors to recombinantly produce MOG IgG1, IgG2, IgG3, IgG4, and Fc mutant (FcMt) mAbs. (A) Sandwich ELISAs indicate binding of MOG IgG1, IgG2, IgG3, and IgG4 mAbs at 10 μg/mL to commensurate subclass-specific antibodies. Serial dilutions of the 4 MOG subclass mAbs, the MOG FcMt mAb, and the AChR IgG1 mAb were tested for MOG binding and effector functions. (B) MAb binding to MOG was quantified as ΔMFI using a live flow cytometry MOG-CBA. (C–F) MAC formation and death of (C and D) MOG+ and (E and F) MOG– cells in the CDC assay. (G and H) Phagocytosis and MOG+ cells out of total HEK cells in the ADCP assay. Each experiment was performed at least 2 times in duplicate. In B–H, each dot represents the average of duplicates.