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. 2023 Jul 14;12:e89702. doi: 10.7554/eLife.89702

Figure 5. fkh-8(vlc43) null mutants display morphological defects in cilia.

Integrated reporters unaffected in fkh-8 mutant are used to label the cilia of several distinct subpopulations of ciliated neurons. CEP: otIs259(dat-1::gfp); ADF: zdIs13(tph-1::gfp); AWB: kyIs104(str-1::gfp); AWA: pkIs583(gpa-6::gfp). Panels show representative images from three animals in wild type and fkh-8(vlc43) mutant backgrounds. Cilium length of CEP and AWB neurons is significantly reduced in the absence of FKH-8 whereas ADF cilia length is increased. Depth of AWA cilium arborization is significantly reduced in fkh-8(vlc43) null mutants. Each dot in the graphs represents measures for a single cilium. Mean and standard error are represented. See Figure 5—figure supplement 1 for cilia morphology analysis in worms immobilized in polystyrene beads and Figure 5—source data 1 for raw data and statistics. CEP and AWB n=40; ADF n=32; AWA n=7.

Figure 5—source data 1. Raw quantification data of cilia morphology analysis depicted in Figure 5 and Figure 5—figure supplement 1.

Figure 5.

Figure 5—figure supplement 1. fkh-8(vlc43) null mutants display morphological defects in cilia using physical immobilization with polystyrene beads.

Figure 5—figure supplement 1.

Integrated reporters unaffected in fkh-8 mutant are used to label the cilia of several distinct subpopulations of ciliated neurons. CEP: otIs259(dat-1::gfp); ADF: zdIs13(tph-1::gfp); AWB: kyIs104(str-1::gfp); AWA: pkIs583(gpa-6::gfp). Panels show representative images from wild type and fkh-8(vlc43) mutant backgrounds. Cilium length of CEP ADF and PHB is significantly reduced in the absence of FKH-8, whereas AWB cilia length is unaffected. Mean and standard error are represented. Please note that different fixation methods are used compared to Figure 5. CEP, AWB: n≥50, ADF: n≥30, PHB: n≥28.