FIG. 2.

Kinetic analysis of the apoptosis levels in cultures of PBMCs from PL, AL, and NI cows. (A) PBMCs were cultured for 1, 6, 14, and 24 h and processed for detection of DNA strand breaks by the TUNEL procedure. Data are mean values from two independent experiments performed in duplicate. SD, standard deviation. The statistical evaluation of the differences between NI, AL, and PL animals was performed with a Student t test. N.S., not statistically significant. (B) Mean values were calculated from the data presented in panel A, according to the stage of the disease. These values are schematically represented for a kinetic analysis after 1, 6, 14, and 24 h of culture.