Figure 6.

Deficits in V̇o₂, V̇co₂, energy expenditure, and CHO oxidation remain following 4-weeks recovery from FOLFOX. A: study design. Male C57BL/6J mice were administered four cycles of either PBS or FOLFOX and allowed to recover for 4 wk after the last injection. Mice were individually housed in CLAMS metabolic cages for 5 days before tissue collection. Body mass (B) and body fat percentage (C) at Post (week 10). D: food intake of individually housed mice during CLAMS measurements. Cage activity (E) and relative (normalized to kg body weight) V̇o₂ (F), V̇co₂ (G), and energy expenditure (H) expressed as the average of the light cycle (0600–1700) and dark cycle (1800–0500). CHO oxidation (I) and metabolic flexibility (J). Lipid oxidation (K) and metabolic flexibility (L). PBS: n = 14, FOLFOX: n = 12. Data are presented as means ± SE. Data are analyzed using unpaired t test (B–D, J, L) or two-way repeated-measures ANOVA with post hoc analysis (E–I, K). Statistical significance set to P < 0.05. Bold and italic values denote significance. *Different from PBS; #main effect of time/cycle; $main effect of FOLFOX. CHO, carbohydrate; CLAMS, Comprehensive Laboratory Animal Monitoring System; FOLFOX, 5-fluorouracil, leucovorin, oxaliplatin; V̇co₂, carbon dioxide production; V̇o₂, oxygen consumption.