Skip to main content
. 2023 Jun 28;325(2):E132–E151. doi: 10.1152/ajpendo.00096.2023

Figure 7.

Figure 7.

Skeletal muscle COXIV enzyme activity and metabolic signaling following 4 wk recovery from FOLFOX. A: COXIV enzyme activity in soleus and plantaris skeletal muscle. B: representative Western blot images with Coomassie blue protein stain, dashed line represents different areas of the same blot. Red gastrocnemius skeletal muscle total protein expression of AMPK, ULK1, and rpS6 (C). D: phosphorylated to total ratio of AMPK (T172), ULK1 (S555), ULK1 (S757), and rpS6 (S240/244). E: puromycin incorporation with representative Western blot and Coomassie blue protein stain, dashed line represents different areas of the same blot. F: protein expression of LC3BI, LC3BII, and the LC3BII/I ratio. G: Spearman r correlation between LC3BII/I and CHO oxidation in FOLFOX mice at 4 wk recovery. PBS: n = 11, FOLFOX: n = 11 or 12. Data are presented as means ± SE. Data are analyzed using unpaired t test and correlation with Spearman r correlation coefficient. Statistical significance set to P < 0.05. Bold and italic values denote significance. *Different from PBS. AMPK, AMP-activated protein kinase; COXIV, cytochrome c oxidase; FOLFOX, 5-fluorouracil, leucovorin, oxaliplatin; LC3B, microtubule-associated protein 1 light chain 3B; rpS6, ribosomal protein S6; ULK1, unc-51-like kinase 1.