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. 2023 Jul 31;7(3):036104. doi: 10.1063/5.0141289

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FIG. 5. — Overexpressed TET1s alleviated OSS-suppressed endothelial F-actin polymerization. (a) HUVECs were transfected with TET1-overexpressed adenovirus or control adenovirus and loaded with OSS for 48 h. Immunofluorescence staining for F-actin (green) and nuclei (blue) in HUVECs. (b) Statistical analysis of nuclear ellipticity (ratio of major axis/minor axis of nucleus) as shown in (a) (n = 100). (c) Statistical analysis of the relative fluorescence intensity of actin as shown in (a) (n = 7). (d) Statistical analysis of polymetric actin ratio (ratio of polymer actin/total actin) as shown in (a) (n = 7). All data were presented as mean ± SD. ns means no significant difference; ^** P < 0.01.