Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 31;7(3):036104. doi: 10.1063/5.0141289

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Author(s).

All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

FIG. 9. — Overexpression of TET1s in HUVECs promoted sFRP-1 promoter demethylation. (a), (b), (d), and (e) HUVECs were transfected with TET1-overexpressed adenovirus or control adenovirus, and loaded with OSS for 48 h. (a) and (b) Dot-blot assay detected the level of global 5hmC in HUVECs (n = 4). (c) The distribution of CpG island sites within 2000 bp of the sFRP-1 promoter region was predicted by MethPrimer2.0. Island1, 160 bp in length, located at 1454-1613; Island2, 115 bp in length, located at 1831-1945. (d) DNA immunoprecipitation-qPCR tested the levels of 5 mC in two CpG island (n = 4). (e) Pyrosequencing assay analyzed the local 5hmC levels in two CpG island. All data were presented as mean ± SD. ns means no significant difference; ^** P < 0.01 and ^*** P < 0.001.