FIG. 5.

Western blot analysis of polyprotein processing of immature viral preparations in the presence of increasing concentrations of diamide following removal of the protease inhibitor. Each preparation of immature virus, obtained as described in Materials and Methods, was incubated in medium at 37°C for 20 h in the presence of the indicated concentrations of diamide. The first lane (U) for each viral preparation contained the unprocessed virus preparation following removal of the protease inhibitor. Protease activity was stopped by the addition of 10 μM protease inhibitor and SDS sample buffer. Blots were probed with a combination of anti-p17 and anti-p24 monoclonal antibodies. The positions of the viral proteins are indicated to the right of the blots. (Top panel) wild-type and double-mutant viral preparations; (bottom panel) C67A and C95A viral preparations.