Fig. 1.

Impact of DON on transepithelial electrical resistance (TEER) in IPEC-1. Cells were grown on inserts and TEER was measured on day 10 (= 0 h/start of DON-application), 24 h, 48 h, and 72 h after DON-application (in ng/mL). TEER-values are expressed in k*cm². Data are given as means (± SEM) of at least 8 experiments. We observed significantly increased TEER values in the CON-group (0 ng/mL DON) but also in the DON-treatment groups 50 ng/mL ap/bl and 200 ng/mL ap/bl after 48 h and 72 h of DON-application compared to the initial values of the corresponding insert (0 h). No differences were found in the group of 2000 ap compared to 0 h. In contrast, incubation of DON 2000 ng/mL bl resulted in significantly decreased TEER after 24 h and a value of 389 Ω*cm² was observed after 72 h. (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001)