Table 2.
Analysis of RNA expression in vivo and in vitro The in vivo RNA levels of the DON-fed group (concentration in ng/mL) were compared with the CON-fed group (= 100%). In the comparison of in vitro RNA levels, IPEC-1 CON group was used as reference and was set to 100%. (T = trend; nd = not detectable)
| Gene |
Jejunal enterocyte (DON-fed vs. CON-fed) |
IPEC-1 50 ap |
IPEC-1 50 bl |
IPEC-1 200 ap |
IPEC-1 200 bl |
IPEC-1 2000 ap |
IPEC-1 2000 bl |
|---|---|---|---|---|---|---|---|
| GAPDH | 151.62 | 130.63 | 124.84 | 137.82 | 125.62 | 85.01 | 102.03 |
| COX5B | 209.28* | 121.01 | 102.12 | 109.58 | 102.18 | 106.44 | 148.09 |
| MHCII | 232.39*** | nd | nd | nd | nd | nd | nd |
| PHB | 92.09 | 119.34 | 121.22 | 140.96 | 132.36 | 200.92 | 317.57** |
| SLC7A11 | 215.74 T | 144.23 | 115.21 | 134.29 | 107.50 | 109.17 | 31.79 |
| TRIC | 201.68* | 96.40 | 86.11 | 91.3 | 91.3 | 87.05 | 90.29 |
| TXNIP | 99.62 | 116.62 | 121.22 | 121.87 | 119.97 | 134.41 | 78.63 |