Figure 3.

Longitudinal effects of antibiotic treatment upon the gut microbiota of mice. (a) Shannon H diversity was determined for each sample as a measure of diversity for mice treated with afabicin or comparators (clindamycin, linezolid or moxifloxacin). (b) Rarefied Chao1 was calculated as a measure of sample richness for mice treated with afabicin, or comparators. Note, rarefied Chao1 was only determined for samples with library size >10 000 reads. (c) Relative abundance percentages for major bacterial families from the murine gut microbiota. For (a) and (b), statistical significance was determined using a mixed-effects model with Dunnett’s multiple comparisons test relative to Day −2 (baseline), * P < 0.05, ** P < 0.01.