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. 2023 Aug 1;13:12491. doi: 10.1038/s41598-023-38828-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antinociception index obtained from the hot plate assay after i.c.v injection of Protonectin-F (16 nmol) or morphine (16 nmol). Control group was treated with vehicle alone. All experimental groups were treated for five consecutive days and the antinociception was measured 120 min after injection. Data were analyzed by Two-Way ANOVA followed by Tukey’s post-hoc test. (*) statistically significant differences (p < 0.05) are indicated when compared to vehicle control, (#) statistically significant differences (p < 0.05) are indicated when compared to morphine group.