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. 2023 Aug 1;6:800. doi: 10.1038/s42003-023-05165-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Heat map of protein abundance (log₂) of proteins identified in each biological replicate (veh-, Ca ion- and SKOV-3-EVs), as identified by LC–MS/MS. b Volcano plots showing average fold changes for protein abundance (log₂) of proteins identified in veh-EVs compared to (i) Ca ion-EVs and (ii) SKOV-3-EVs. P-values were calculated using student’s T-test, and significantly changing proteins (p-value ≤ 0.05 and fold change >2) in veh-EVs are highlighted in red, while significantly changing proteins in Ca ion- and SKOV-3-EVs are highlighted in blue. c Western blot analysis confirming the enrichment of MS-identified proteins NID1, TSG-6, LAMC1, PAPP-A, CD81 and β-actin (β-ACT) in veh-EVs compared with Ca ion-EVs. CNX was solely present in CPC lysate (CL). Complete blots of β-ACT, PAPP-A and NID1 are displayed in Supplementary Fig. 10. d Venn diagram showing number of proteins with >2-fold significant enrichment (p ≤ 0.05) in veh-EVs compared to Ca ion- and SKOV-3-EVs, and overlap between those two populations. e Gene ontology analysis using PANTHER of enriched biological processes for the 105 overlapping proteins, depicting number of identified proteins in each group, ranked on smallest corrected p-value (−log₁₀(FDR)).