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. 2023 Aug 1;14:4605. doi: 10.1038/s41467-023-40395-7

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a PR interacting proteins in decidual HESCs by RIME. b Immunofluorescence (IF) of TRIM28 (magenta) and PR (green) in pre-decidual and decidual HESCs. c TRIM28 and PR proximity (red dots, arrow pointed) in siNT and siTRIM28 treated pre-decidual and decidual HESCs by Proximity Ligation Assay. d Co-immunoprecipitation of TRIM28 and PR in the decidual HESCs and PR/TRIM28 overexpressed HELA cells. The IF was repeated in three primary cell line. The proximity and co-IP in decidual HESCs were repeated in two primary cell lines. The co-IP in the overexpressed HELA cells was repeated for three independent experiments. All repeats show similar results.