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. 2023 Aug 1;14:4605. doi: 10.1038/s41467-023-40395-7

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

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Fig. 2 — a Edu labeled cell proliferation, migrated cells in the transwell assay, and brightfield images of pre-decidual and decidual HESCs. Yellow line circles the cells. b Comparison of siPGR and siTRIM28 mediated transcriptomic changes in the hormone stimulated decidual HESCs. c The heatmap of PR and TRIM28 binding peaks in decidual HESCs by CUT&RUN. d The overlapped peaks of TRIM28 and PR, the associated common DEGs altered by TRIM28 and PGR knockdown in the decidual HESCs. Genome browser display of PR and TRIM28 binding peaks, and the CUT&RUN PCR of PR binding activity at PTGFR e and SULF2 f promoter in the siNT or siTRIM28 treated decidual HESCs. N = 500,000 cells examined over three independent experiments and repeated in two primary cell lines with similar results. Two-sided student’s t test. The bight image, cell proliferation, and proliferation were repeated in three primary cell lines with similar results.