Fig. 5. TRIM28 deletion inhibited ERα activity.
a Immunofluorescence (IF) of PR and ERα. b The overlapped binding peaks of TRIM28 and ERα, the associated common DEGs and top altered pathways between TRIM28d/d mice and E2 treated ovariectomized wildtype mouse uterus. c The genome browser of ERα, TRIM28 and H3K27AC binding peaks at Pgr. Blue line is the Chromatin loops predicted by HiC. d The ChIP-qPCR of ERα binding activity at the promoter and 3′ peak enhancer. Two-sided student’s t test. *p < 0.05. N = 6 biologically independent samples per group. Rep: replicate. The IF was repeated in three different mice per group with similar results.