Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
10× PBS
| Reagent | Final concentration | Amount |
|---|---|---|
| NaCl | 1.37 M | 8 g |
| KCl | 26.8 mM | 0.2 g |
| Na2HPO4.2H2O | 97.75 mM | 1.74 g |
| KH2PO4 | 17.6 mM | 0.24 g |
| Double distilled water | Up to 100 mL | |
| Total | 100 mL |
When 10XPBS is diluted to 1×, the pH should be 7.4. If required, adjust the pH with HCl and check using a digital pH meter. For prolonged storage, autoclave the 10× PBS and then store it at 25°C–28°C. You can store this buffer for 4–6 months in a 250 mL glass bottle.
