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. 2023 Aug 1;14:4620. doi: 10.1038/s41467-023-40327-5

Fig. 4. CAND1 controls the ubiquitination and degradation of fatty acid β-oxidation gene ACAA2.

Fig. 4

a Volcano plot of Cullin1-binding protein in L02 induced by PA with NC or siCAND1. b Heatmap of deregulated binding proteins in L02 cells induced by PA after knockdown of CAND1. c Molecular docking of Cullin1, FBXO42 and ACAA2. d Immunoprecipitated with anti-Cullin1 antibody of NC or SI CAND1 groups after PA treatment in AML12, and then immunoblotted with antibodies specific for FBXO42 and ACAA2 (n = 3). e Immunoprecipitated with anti-Cullin1 antibody of NC or SI CAND1 groups after PA treatment in THLE-2, and then immunoblotted with antibodies specific for FBXO42 and ACAA2 (n = 3). f Western blotting of ACAA2 in the livers of WT and cKO mice after 16 weeks of NC or HFD feeding (n = 4). g Ubiquitination of ACAA2 in the livers of WT and cKO mice after 16 weeks of NC or HFD feeding (n = 3). h Western blotting of CAND1 in the livers of WT and cKI mice after 16 weeks of NC or HFD feeding (n = 4). i Ubiquitination of ACAA2 in the livers of WT and cKI mice after 16 weeks of NC or HFD feeding (n = 3). p values obtained via two-tailed unpaired Student’s t tests, one-way ANOVA with Tukey’s multiple comparisons test. The data were shown as means ± SD of independent biological replicates. Source data are provided as a Source data file.