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. 2023 Jul 3;55(7):1413–1423. doi: 10.1038/s12276-023-01023-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Coronal and sagittal MRI of LF. The LF thickness was determined on coronal MRI. B Comparison of LF thickness between the LFH group and the non-LFH group (n = 20). C Representative images of H&E staining and EVG staining of the LF samples from the two groups (n = 8). EVG staining (collagen fibers were stained pink, while elastic fibers were stained black). The scale bar indicates 100 μm. D Comparison of the percentage of collagen fibers and elastic fibers between the two groups (n = 8). E Comparison of LF fibrosis scores between the two groups (n = 8). F Western blot analysis of collagen I, collagen III, α-SMA and fibronectin protein expression in LF samples from the two groups. GAPDH was the loading control (n = 6). G Quantitative analysis of collagen I, collagen III, α-SMA and fibronectin protein expression in LF samples from the two groups (n = 6). H Representative images of immunohistochemical staining of collagen I, collagen III, α-SMA and fibronectin in LF samples from the two groups (n = 8). The scale bar indicates 100 μm. Data are presented as the means ± SDs. ns, no significance; *p < 0.05; **p < 0.01; ***p < 0.001.