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. 2023 Jul 3;55(7):1413–1423. doi: 10.1038/s12276-023-01023-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Calcein-AM/PI double staining of LF cells after intervention with different concentrations of DCN (green, living cells; red, nuclei of dead cells). The scale bar indicates 400 μm. B Quantitative analysis of the percentage of living cells (six random fields of view for each well). C CCK-8 assay of LF cells showed that DCN suppressed the proliferation of LF cells, especially when the concentration of DCN was 100 nM and 200 nM (n = 6). Data are presented as the means ± SDs. ns no significance; *p < 0.05; **p < 0.01; ***p < 0.001.