Skip to main content
. 2023 Jul 25;12(1):A0128. doi: 10.5702/massspectrometry.A0128

Fig. 5. MSI results and TPP-DA determination in mouse brain St. DA-d4 was used as the IS for DA. (A) MSI results are MS images obtained after the calculation of TPP-DA/TPP-DA-d4 using the analysis software IMAGEREVEAL (Shimazu, Kyoto, Japan). (B) Quantitative values for ELISA kits. This ELISA kit is a competitive ELISA. Two-dimensional fitting was used because it is generally recommended to use a four-parameter logistic curve, as the competing method results in an inverse sigmoid curve. (C) Quantitative values for MALDI-MSI with IS. DA, dopamine hydrochloride; DA-d4, dopamine-d4 hydrochloride; ELISA, enzyme-linked immunosorbent assay; IS, internal standard; MALDI, matrix-assisted laser desorption/ionization; MS, mass spectrometry; MSI, mass spectrometry imaging; St, striatum; TPP, triphenylpyrylium tetrafluoroborate.

Fig. 5. MSI results and TPP-DA determination in mouse brain St. DA-d4 was used as the IS for DA. (A) MSI results are MS images obtained after the calculation of TPP-DA/TPP-DA-d4 using the analysis software IMAGEREVEAL (Shimazu, Kyoto, Japan). (B) Quantitative values for ELISA kits. This ELISA kit is a competitive ELISA. Two-dimensional fitting was used because it is generally recommended to use a four-parameter logistic curve, as the competing method results in an inverse sigmoid curve. (C) Quantitative values for MALDI-MSI with IS. DA, dopamine hydrochloride; DA-d4, dopamine-d4 hydrochloride; ELISA, enzyme-linked immunosorbent assay; IS, internal standard; MALDI, matrix-assisted laser desorption/ionization; MS, mass spectrometry; MSI, mass spectrometry imaging; St, striatum; TPP, triphenylpyrylium tetrafluoroborate.