Skip to main content
. 2023 May 29;114(8):3176–3189. doi: 10.1111/cas.15852

FIGURE 1.

FIGURE 1

TRIM29 is hypomethylated and its expression is elevated in nasopharyngeal carcinoma (NPC). (A) mRNA levels of candidate genes in nasopharyngeal epithelial cell line NP69 and NPC cell lines were verified with real‐time PCR. (B) The mean percentage of methylation (%) and representative pyrograms in the TRIM29 gene region analyzed by pyrosequencing in NP69 and NPC cell lines. (C) The mean percentage of methylation (%) by pyrosequencing and representative pyrograms in the TRIM29 gene region in nasopharyngeal epithelial (normal) and nasopharyngeal carcinoma tissues (NPC); graph shows the combined data. (D) Expression of TRIM29 in NPC cell lines was measured by western blot (WB) analysis. (E) Expression of TRIM29 in normal nasopharyngeal tissues (N) and NPC tissues (T) was detected by WB analysis. (F) Real‐time PCR and WB analysis showed that treatment with 5‐aza‐deoxycytidine (5‐Aza) induced TRIM29 mRNA and protein expression in NP69 cells. (G) Real‐time PCR and WB analyses showed that treatment with S‐adenosylmethionine (AdoMet, 200 μM) reduced TRIM29 mRNA and protein expression in CNE2 and HONE1 cells. *p < 0.05**p < 0.01; ***p < 0.001.