TABLE 1.
Summary of culture methods.
| Our conventional method | Our improved method | Nanki et al. 13 | Yan et al. 14 | |
|---|---|---|---|---|
| Sampling method | ||||
| Site | Inside the tumor boundary | Both sides of the tumor boundary | NS | NS |
| Number of tissue pieces | 1–2 | 3–4 | NS | NS |
| Area (mm2)/Depth (mm) | 50–150/2–3 | 100–200/3–5 | NS | NS |
| Matrix material | Matrigel | Matrigel and collagen‐I, separately | Matrigel | Matrigel |
| Medium composition | ||||
| Growth factor | EGF, FGF2, FBS | EGF, FGF2, FBS | EGF, FGF10 | EGF, FGF10, FBS (as CM) |
| Stem cell niche factor | – | L‐WRN CM | Afamin‐Wnt3a CM, RSPO1, Noggin | Wnt3a CM, RSPO1 CM, Noggin CM |
| Inhibitor | SB431542, Y27632 | SB431542, Y27632 | A83‐01 | A83‐01, Y27632 |
| Other supplements | B27, NECA | B27, NECA | B27, Gastrin, NAC | B27, Gastrin, NAC |
| Selection procedure for cancer cell enrichment | No selection | No selection | +Nutlin‐3, –A83‐01/+TGF‐β, –EGF/–FGF10, or single‐cell dissociation | Manual picking or +Nutlin‐3 |
| Success rate | 25% (18/71) (95% CI, 15%–35%) | 88% (29/33) (95% CI, 77%–99%) | 75% (44/59) | >50% |
Note: Two representative methods reported previously are also shown as references.
Abbreviations: −, no or withdrawal from the culture medium; +, addition to the culture medium; CI, confidence interval; CM, conditioned medium; EGF, epidermal growth factor; FBS, fetal bovine serum; FGF, fibroblast growth factor; NAC, N‐Acetyl‐l‐cysteine; NECA, 5’‐N‐ethylcarboxamine adenosine; NS, not specified; RSPO1, R‐spondin 1; TGF‐β, transforming growth factor beta.