Prognostic impact of tertiary lymphoid structures in ovarian cancer
(A) Summary of prognostic potential of immune cell subpopulations in TCGA ovarian cancer cohort. Hazard ratio of progression-free survival is plotted on the x axis, in a univariate Cox regression model (light blue) and multivariable Cox regression model (red); n = 345. Statistically significant association is shown in darker colors.
(B) Correlation between B cells and TLS markers in TCGA cohort.
(C) TLS abundance across cancer types in TCGA cohort.
(D) Immunohistochemistry staining of CD20 in TLS-low (left) and TLS-high (right) ovarian tumor tissues. Scale bar indicates 200 μm.
(E and F) Kaplan-Meier plots of TLS and progression-free survival in (E) HH cohort and (F) TCGA cohort.
(G) Overview of the study. Whole-exome sequencing to define mutational and copy-number profiles; immunohistochemistry for immune markers and radiomics profile are collected from HH cohort; two public single-cell RNA-sequencing datasets are reanalyzed. The TLS signature is derived from the B lineage from MCPcounter.