FIG. 6.

(A) Schematic diagrams of plasmids used to synthesize monocistronic RNAs. The lines indicate PSIV sequences and the capsid-coding regions are shown as shaded boxes. Triangles represent the location of the T7 promoter. The 5′-terminal nucleotide sequence of the RNA transcribed from the plasmid pT7-6173 is shown. Italic and roman letters indicate the vector and PSIV sequences, respectively. +1 represents the transcription start site for T7 RNA polymerase. The CUU initiation codon is underlined. For pT7-6184TAA and pT7-6190ATG, only mutated codons are shown. (B) In vitro translation products of uncapped and capped RNAs synthesized from pT7-5800, pT7-6173, pT7-6184TAA, and pT7-6190ATG. The position of the translation products (capsid protein) is indicated on the left of the panel. (C) Comparison of the molecular masses of the translation products from pT7-5800 and pT7-6173. To show the difference in mobility, the same amounts of products were electrophoresed.