Table 1.

Plasmids and oligonucleotides within this study.

Plasmids	Description
pA-SRAI	E. coli dxs, dxr, ispA, and idi genes under PBAD promoter control were cloned in a plasmid of p15A origin and a chloramphenicol resistance gene
pWA-EBI, EIB, BEI, BIE, IEB, IBE	D. wulumuqiensis R12-derived enzyme genes (crtE (E), crtB (B), crtI (I)) were cloned in various orders and transcribed under a synthetic promoter (BBa_J23118) control. The plasmid containing the three genes harbored ColE1 origin and an ampicillin resistance gene.
Primers	Oligonucleotide sequence1
crtE-F crtE-R	5'ATGCCTCGAGGAAGTGTACCGGAGAAGTGGC 3' 5'ATGCGGATCCATGCATGTCGACTATTTTTTCCTACTCGCATCCGC 3'
crtB-F crtB-R	5'ATGCCTCGAGTGAACGTGACGGAATTTTCGC 3' 5'ATGCGGATCCATGCATGTCGACGTGAACCTCTGAACATGTAGAAG 3'
crtI-F crtI-R	5'ATGCCTCGAGGCACCTTCTTCCCCTTTCTCTC 3' 5'ATGCGGATCCATGCATGTCGACCGTCCGTATGGGTTTTGGACAA 3'
Dxs-F Dxs-R	5'TAAAAGGAGACCCGGGATATGAGTTTTGATATTGCCAAATACCCGACCC 3' 5'CAGGGGCCTATTAATACTTATTGTTTATGCCAGCCAGGCCTTGATTTTGGCTTCC 3'
Dxr-F Dxr-R	5'AGTATTAATAGGCCCCTGATGAAGCAACTCACCATTCTGGGCTC 3' 5'GCGTTTTTTATTCCCTGACAGGGTTCAGCTTGCGAGACGCATCACCTCTTTTCTGGC 3'
ispA-F ispA-R	5'TCAGGGAATAAAAAACGCATGGACTTTCCGCAGCAACTCGAAGCCTGCG 3' 5'GCTGCCACTCCTGCTATACTCTTATTTATTACGCTGGATGATGTAGTCCGCTAGC 3'
Idi-F Idi-R	5'TATAGCAGGAGTGGCAGCATGCAAACGGAACACGTCATTTTATTGAATGC 3' 5'TTTGATGCCTGGCTCGAGTTATTTAAGCTGGGTAAATGCAGATAATCGTTTTC 3'

¹Restriction enzyme site are underlined. XhoI (CTCGAG), BamHI (GGATCC)/SalI (GTCGAC)