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Pathological abnormalities of brain tissue and neurons restored by LTC, manifested from HE and Tunel staining and NeuN IHC assay. (A) HE staining, black arrows indicate vacuolation and karyopyknosis of the neuronal cell in the representative photomicrographs; (B) NeuN IHC assay, brown arrows show the positive staining of the neuronal cell in the representative photomicrographs; (C) TUNEL staining, brown arrows indicate apoptotic vascular cells in the representative photomicrographs.
