Table 2. Muramidase activity (OD-drop) of TsGH24 with and without the CWBD.

CWBD-GH24 was dosed at 6.8 µg ml⁻¹ and GH24-CD was dosed at 4.5 µg ml⁻¹ to compensate for the difference in molecular weight. The molecular weight of CWBD-GH24 is 26 kDa and that of GH24-CD is 18 kDa. CWBD was dosed at 4.5 µg ml⁻¹. The OD-drop at pH 6.0 was measured after 60 min.

TsGH24 construct	Amino-acid residues of NCBI ID ON783686	OD-drop
CWBD-GH24	1–245	0.50
GH24-CD	81–245	0.17
CWBD	1–73	−0.03