EMSA of the HERV-K LTR subfragment from bp 1 to 167 as a probe with nuclear extracts of the cell lines indicated. Lanes: O: no protein extract added; N, no competitor added; S, unlabeled LTR fragment from bp 1 to 167 as specific competitor; U, unlabeled oligonucleotide containing the Sp1 binding site as a nonspecific competitor. All bands are competed specifically. One teratocarcinoma-specific (T1) and two hepatocarcinoma-specific (H1 and H2) complexes are discernible; C1, C2, and C3 are common to all cell lines.