Fig. 2.

circNIPBL promotes the proliferation, migration, and invasion of BCa cells in vitro and in vivo. (A–D) qRT-PCR was used to assess the expression level of circNIPBL and NIPBL in circNIPBL knockdown (A, B), circNIPBL overexpression (C, D), and paired control BCa cells. (E) Representative images and quantification of Wound healing assay of UM-UC-3 cells treated with circNIPBL-downregulated or -overexpressing. Scale bar = 100 μm. (F) Representative images and quantification of Transwell migration and Matrigel invasion assays of UM-UC-3 cells treated with circNIPBL-downregulated or -overexpressing. Scale bar = 100 μm. (G-I) Representative bioluminescence images (G) and quantification (H, I) of in tail-vein injection model. Scale bar = 50 μm and 20 μm. (J) The ratio of lung metastasis was calculated for all groups (n = 12 per group). The statistical difference was assessed with one-way ANOVA followed by Dunnett tests in A, B, E and F; and the two-tailed Student t test in C, D, E, F, H and I; and the χ² test in J. Error bars show the SD from three independent experiments. *p < 0.05 and **p < 0.01. H&E, hematoxylin and eosin