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. 2023 Aug 2;42:191. doi: 10.1186/s13046-023-02757-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — circNIPBL attenuates miR-16-2-3p-mediated Wnt signaling suppression. (A) Schematic illustration showing the downstream targets of miR-16-2-3p as predicted by the TargetScan, Pictar and miRDB databases. (B) KEGG pathway enrichment analysis with PANTHER database of BCa. (C) qRT-PCR analysis of Wnt signaling pathway-related genes in circNIPBL overexpressing BCa cells. (D) Schematic illustration showed the alignment of miR-16-2-3p with Wnt5a and the mutagenesis nucleotides were indicated by the red portion. (E) The luciferase activities of the Wnt5a-wt plasmid or Wnt5a-mut plasmid quantified following transfecting control mimic or miR-16-2-3p mimic into BCa cells. (F, G) qRT-PCR analysis of the impact of circNIPBL knockdown (F) or circNIPBL overexpression (G) on Wnt5a expression in UM-UC-3 cells. (H, I) Representative images (H) and quantification (I) of Western blotting assay of Wnt5a in indicated UM-UC-3 cells. (J-M) Western blotting assay showed the expression levels of Wnt5a and β-catenin after circNIPBL knockdown (J, K) or circNIPBL overexpression (L, M) in UM-UC-3 cells. The statistical difference was assessed with one-way ANOVA followed by Dunnett tests in F, I and K; and the two-tailed Student t test in C, E, G and M. Error bars show the SD from three independent experiments. *p < 0.05 and **p < 0.01