Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 2;42:191. doi: 10.1186/s13046-023-02757-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — circNIPBL facilitates the expression level of ZEB1. (A) qRT-PCR analysis showed that the expressions of downstream targets of Wnt signaling pathway in circNIPBL overexpressing BCa cells. (B) qRT-PCR analysis of ZEB1 expression in indicated UM-UC-3 cells. (C, D) Representative images (C) and quantification (D) of Western blotting assay of ZEB1 in indicated UM-UC-3 cells. (E) qRT-PCR analysis of ZEB1 expression in indicated UM-UC-3 cells. (F-I) Representative images and quantification of Western blotting assay of ZEB1 in UM-UC-3 cells transfected with miR-16-2-3p inhibitors (F, G) and mimics (H, I). (J-M) Representative images and quantification of Western blotting assay of N-cadherin, E-cadherin, ZEB1 after circNIPBL knockdown (J, K) or circNIPBL overexpression (L, M) in UM-UC-3 cells. (N, O) The expression of N-cadherin and E-cadherin was detected by IF assay in circNIPBL knockdown (N) or circNIPBL overexpression (O) UM-UC-3 cells. Scale bar = 5 μm. (P, Q) Representative images (P) and quantification (Q) of Western blotting assay of N-cadherin and E-cadherin in indicated UM-UC-3 cells. (R) The expression of N-cadherin and E-cadherin was detected by IF assay in indicated UM-UC-3 cells. Scale bar = 5 μm. The statistical difference was assessed with one-way ANOVA followed by Dunnett tests in B, D, E, K and Q; and the two-tailed Student t test in A, G, I and M. Error bars show the SD from three independent experiments. *p < 0.05 and **p < 0.01