TABLE 5.

Lymph node lymphocyte populations as determined by flow cytometry

SIVPBjnef-deleted-virus-immunized macaque							SIV239nef-deleted-virus-immunized macaque
Animal no.	Virus isolation	Percentage of total lymphocytesc				CD4:CD8 ratio	Animal no.	Virus isolation	Percentage of total lymphocytesc				CD4:CD8 ratio
		CD20+	CD2+	CD4+	CD8+				CD20+	CD2+	CD4+	CD8+
2370 Prea	−	19	73	45	27	1.67	343 Pre	+	9	87	57	28	2.04
2370 Postb	+	21	66	41	28	1.46	343 Post	+	14	82	46	28	1.64
2394 Pre	+	17	78	61	18	3.39	344 Pre	+	NDd	ND	ND	ND
2394 Post	+	22	69	55	18	3.06	344 Post	+	18	68	35	27	1.30
2574 Pre	−	27	66	47	21	2.24	348 Pre	+	24	68	45	26	1.73
2574 Post	+	37	49	35	21	1.67	348 Post	+	28	67	30	29	1.03
2580 Pre	−	26	66	48	20	2.40	354 Pre	+	26	64	43	28	1.54
2580 Post	−	29	60	45	20	2.25	354 Post	+	23	50	35	30	1.17
2581 Pre	−	18	78	58	20	2.90	356 Pre	+	18	77	46	30	1.53
2581 Post	+	21	73	57	20	2.85	356 Post	+	34	58	22	31	0.71
1159 Pre	−	15	81	63	18	3.50	1060 Pre	−	11	83	54	30	1.80
1159 Post	+	40	59	0.7	49	0.01	1060 Post	+	21	59	1.6	54	0.03
1235 Pre	−	16	80	60	20	3.00	1128 Pre	−	14	79	58	22	2.64
1235 Post	+	37	55	0.4	55	0.01	1128 Post	+	29	49	1.3	46	0.03

^aPre, lymph node collected prior to SHIV_89.6PD challenge. 

^bPost, lymph node collected 2 weeks after SHIV_89.6PD challenge. 

^cValue equals the percentage of total lymphocytes gated by forward scatter versus side scatter differentiation. 

^dND, not done.