Table 1.
Chromatographic conditions and Parameters of system suitability of the proposed HPLC method for the determination of PAR and DAN
| Chromatographic conditions | Column | Hypersil C18 column | |
|---|---|---|---|
| Mobile phase | 40:60 (v/v) of ethanol: water, 0.2% TEA at pH 4.5 0.02 and 0.8 mL/min flowrate | ||
| Detection | UV detection 290 nm | ||
| Parameters of system suitability | |||
| Parameter | PAR | DAN | |
| No of theoretical plates, N | 1936 | 4500 | |
| Selectivity factor, α | 12.66 | ||
| Resolution, Rs | 8.75 | ||
| Retention time (tR), min | 2.2 | 5.7 | |
| Tailing factor (T), 5% of the peak height | 0.97 | 1.14 | |
| Asymmetry factor, 10% of the peak height | 1.11 | 1.21 | |
| Run time, min | 6 | ||