Table 4.

Assay results for the determination of PAR and DAN in pure form by the suggested and official USP methods

Compound	Suggested technique			Official USP method [33]
	Concentration taken (µg/mL)	Concentration found (µg/mL)	% Found	% Found
PAR	1.0	0.98	98.00	100.57
	2.0	1.957	97.85	99.11
	5.0	4.911	98.22	100.37
	10.0	9.957	99.57
	20.0	20.066	100.33
	30.0	29.804	99.35
	40.0	40.506	101.27
	60.0	59.823	99.71
	100.0	100.388	100.39
	200.0	199.782	99.89
Mean			99.46	100.02
± S.D			1.13	0.792
t test			0.79 (2.20) *
F test			2.03 (19.38) *
DAN	1.0	0.981	98.10	100.76
	2.0	2.016	100.80	98.88
	5.0	5.026	100.52	100.45
	10.0	9.806	98.06
	20.0	20.116	100.58
	30.0	30.322	101.07
	40.0	39.745	99.36
Mean			99.78	100.03
± S.D			1.28	1.01
t test			0.29 (2.31) *
F test			1.62 (19.33) *

N.B. Each result is the average of three separate determinations

^*The figures between parentheses are the tabulated t and F values at P = 0.05 [32]