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. 2023 Jan 10;15(1):mjad001. doi: 10.1093/jmcb/mjad001

Figure 4.

Figure 4

Combined radiotherapy–RIG-I immunotherapy induces p53 pathway activation and prolongs cell cycle arrest. (A and B) Western blot analysis of phospho-p53 (Ser15), total p53, p21, and PUMA expression after irradiation (2 Gy), transfection of 50 ng/ml 3pRNA, or both in B16 cells at the indicated time points. Actin served as a protein-loading control. (C) Flow-cytometric cell cycle analysis of B16 cells stained with propidium iodide at the indicated time points. Mean ± SEM of n = 2. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; two-way ANOVA.