FIG. 3.

Pathway of Tax responsible for IL-2-independent proliferation of CTLL-2 cells. (A) Expression of Tax proteins in CTLL-2 cells. Cell lysates were prepared from CTLL-2 cells transfected either with vector plasmid (pHβPr.1-neo) or with expression vectors encoding TaxWT or the indicted Tax mutant proteins. The expression of Tax proteins in these lysates was measured by Western blot analysis with anti-Tax antibody (Taxy-8). (B) Cell growth analysis of CTLL-2 cells expressing each Tax mutant. CTLL-2 clones expressing the indicated Tax mutant were cultured in the absence of IL-2, and cell growth was measured by trypan blue staining.