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. Author manuscript; available in PMC: 2024 May 19.
Published in final edited form as: ACS Chem Biol. 2023 Apr 24;18(5):1136–1147. doi: 10.1021/acschembio.2c00939

Figure 7.

Figure 7.

Env8 binding pocket mutants binding to Cbl derivatives. A) A20 mutants were analyzed for binding to CNCbl, 8, and 9 by SHAPE. Nucleotide A20 and region L5 are shown in a representative gel and are quantified (n=2). B) Determination of KD by fluorescence induction upon CNCbl-5xPEG-ATTO590 (top) and PhNO2-Cbl-5xPEG-ATTO590 (bottom) binding to env8 A20 mutants. C) Same as B, but mutations to regions J6/3 (A68G) and J1/3 (ΔA9, G10U, G12A), alone and in combination with A20G. (n=3–4) Error is represented as SEM.