Figure 8.

In vivo fluorescence reporter assay shows Cbl derivatives are capable of regulating translation of a protein (mNeonGreen) under control of the Cbl riboswitch (n=3). Protein fluorescence is measured on the right y-axis and shown with symbols. Fluorescence fold repression compared to the no ligand condition is measured on the left y-axis and shown with green box-and-whisker plots. 7, shown to have low affinity for env8 by displacement assay (Figure 3), was used as a negative control. K_rel values of the Cbl derivatives from Table 1 are included for reference.