Fig. 1.

Progression of AKI induced by IRI. (A) Schematic diagram of ischemic treatment and sample collection. (B–C) Serum creatinine and BUN levels were determined at different time points (n = 6). (D) PAS (red, glycogen) and Masson staining (blue, collagen) for histological changes after IRI. Long arrows indicate fibrotic area, short arrows represent tubular injury, while arrowheads show the infiltrated inflammatory cells. Scale bar, 80 μm. (E–F) Kidney injury score and fibrotic area were quantified (n = 6). Data are expressed as the means ± SDs for each group. Differences between the two groups were assessed with a two-tailed Student’s t-test. *P < 0.05 versus control; **P < 0.05 versus 3D/7D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)