Fig. 4.

Validation of the proteins involved in peroxisomal fatty acid oxidation in IRI mice. (A–C) Western blotting was used to determine the expression of Acox1, Crot and Amacr in renal cortex during AKI progression, and their levels are presented relative to those of GAPDH (n = 6). Data are expressed as the means ± SDs for each group. Differences between the two groups were assessed with a two-tailed Student’s t-test. *P < 0.05 versus control. **P < 0.05 versus 7 d. (D) Representative immunostaining micrographs and semi-quantified positive area of Acox1 in IRI mice. (E) Oil red O staining and its semi-quantification of the kidneys after IRI. Scale bar, 40 μm. (F) Colocalization of Acox1 and fibrotic markers (α-SMA and collagen I). Scale bar, 160 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)