Fig. 5.

Validation of the proteins involved in peroxisomal fatty acid oxidation in the UUO model. (A) Masson staining for histological changes and IHC staining for α-SMA of kidney sections (n = 6). (B–C) Fibrotic and α-SMA positive areas were quantified (n = 6). (D–E) Western blotting was used to determine the expression of Acox1 in UUO, and their levels are expressed relative to those of GAPDH. (F–H) Representative micrographs show renal Acox1 expression and Oil red O staining in UUO mice, and the percentage of positive area was quantified. Scale bar, 40 μm. Data are expressed as the means ± SDs for each group. Differences between the two groups were assessed with Student’s t-test. *P < 0.05 versus control; **P < 0.05 versus 14 d. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)