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. 2023 Jul 18;13:1164070. doi: 10.3389/fonc.2023.1164070

Figure 3.

Figure 3

PFDN2 promotes cell cycle progression in GC cells. (A) Bubble chart showing Gene Ontology (GO) enrichment analysis of the RNA-sequencing (RNA-seq) data from PFDN2-KD and HGC27-NC cells. (B) GSEA of the RNA-sequencing (RNA-seq) data from PFDN2-KD and HGC27-NC cells. (C) Flow cytometry was used to analyze the cell cycle in HGC27-NC and PFDN2-KD cell lines and in MKN45-NC and PFDN2-OE cell lines. (D) Cell proliferation abilities were determined by EdU staining in HGC27-NC and PFDN2-KD cell lines and in MKN45-NC and PFDN2-OE cell lines. (E) WB analysis of PFDN2, cyclin D1, cyclin E1 and CDK2 in HGC27-NC and PFDN2-KD cell lines and in MKN45-NC and PFDN2-OE cell lines. The protein levels were quantified with ImageJ. **p < 0.01; ***p < 0.001.